Liposomal glycosphingolipids activate natural killer T cell-mediated immune responses through the endosomal pathway

Joe Inoue, Ryuichi Ideue, Daisuke Takahashi, Masahiro Kubota, Yoshio Kumazawa

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Natural killer T (NKT) cells recognize lipid antigens, such as glycosphingolipids (GSLs), via CD1d and contribute to host defense against various pathogens. Here, we demonstrate that GSLs isolated from Sphingomonas bacteria and inserted into liposomes (GSL-liposomes) enhance the activation of NKT cells and dendritic cells (DCs). GSL-liposomes remarkably enhanced the production of IFN-γ from splenocytes in vitro and this enhancement depended on the content of the pH-sensitive lipid dioleoyl-phosphoethanolamine (DOPE) in the liposomes. GSL-liposomes containing DOPE were clearly broken in late endosomes and this may facilitate effective loading of GSLs onto CD1 molecules. Treatment with GSL-liposomes also activated NKT cells and DCs in vivo. Collectively, our results strongly suggest that GSL-liposomes can effectively induce NKT cell-mediated immune responses and may be useful as an immune adjuvant for inducing protective immunity.

本文言語English
ページ(範囲)18-23
ページ数6
ジャーナルJournal of Controlled Release
133
1
DOI
出版ステータスPublished - 2009 1 5
外部発表はい

ASJC Scopus subject areas

  • 薬科学

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