LNGFR+THY-1+VCAM-1hi+ cells reveal functionally distinct subpopulations in mesenchymal stem cells

Yo Mabuchi, Satoru Morikawa, Seiko Harada, Kunimichi Niibe, Sadafumi Suzuki, Francois Renault-Mihara, Diarmaid D. Houlihan, Chihiro Akazawa, Hideyuki Okano, Yumi Matsuzaki

研究成果: Article査読

128 被引用数 (Scopus)

抄録

Human mesenchymal stem cells (hMSCs), which conventionally are isolated based on their adherence to plastic, are heterogeneous and have poor growth and differentiation, limiting our ability to investigate their intrinsic characteristics. We report an improved prospective clonal isolation technique and reveal that the combination of three cell-surface markers (LNGFR, THY-1, and VCAM-1) allows for the selection of highly enriched clonogenic cells (one out of three isolated cells). Clonal characterization of LNGFR+THY-1 + cells demonstrated cellular heterogeneity among the clones. Rapidly expanding clones (RECs) exhibited robust multilineage differentiation and self-renewal potency, whereas the other clones tended to acquire cellular senescence via P16INK4a and exhibited frequent genomic errors. Furthermore, RECs exhibited unique expression of VCAM-1 and higher cellular motility compared with the other clones. The combination marker LNGFR+THY-1 +VCAM-1hi+ (LTV) can be used selectively to isolate the most potent and genetically stable MSCs.

本文言語English
ページ(範囲)152-165
ページ数14
ジャーナルStem cell reports
1
2
DOI
出版ステータスPublished - 2013

ASJC Scopus subject areas

  • 生化学
  • 遺伝学
  • 発生生物学
  • 細胞生物学

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