Logic gate operation by DNA translocation through biological nanopores

Hiroki Yasuga, Ryuji Kawano, Masahiro Takinoue, Yutaro Tsuji, Toshihisa Osaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Logical operations using biological molecules, such as DNA computing or programmable diagnosis using DNA, have recently received attention. Challenges remain with respect to the development of such systems, including label-free output detection and the rapidity of operation. Here, we propose integration of biological nanopores with DNA molecules for development of a logical operating system. We configured outputs "1" and "0" as singlestranded DNA (ssDNA) that is or is not translocated through a nanopore; unlabeled DNA was detected electrically. A negative-AND (NAND) operation was successfully conducted within approximately 10 min, which is rapid compared with previous studies using unlabeled DNA. In addition, this operation was executed in a four-droplet network. DNA molecules and associated information were transferred among droplets via biological nanopores. This system would facilitate linking of molecules and electronic interfaces. Thus, it could be applied to molecular robotics, genetic engineering, and even medical diagnosis and treatment.

本文言語English
論文番号e0149667
ジャーナルPloS one
11
2
DOI
出版ステータスPublished - 2016 2

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

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