Long-lived retrograde fluorescent labeling of corticospinal neurons in the living animal

Takeshi Suzuki, Yoshihiro Abe, Daniel S. McGehee, J. Russel Keath, Hiroshi Yajima, Kamal Sharma, James R. Brorson

研究成果: Article査読

6 被引用数 (Scopus)

抄録

For pathophysiological studies, it is advantageous to label specific neuronal populations in living animals. This study aimed to establish a method for stable and long-lasting fluorescent labeling of corticospinal neurons in the living animal. The two fluorescent dyes Fluoro-Red and Fluoro-Green were injected in the cervical spinal cord of anesthetized newborn rats. After a recovery period, treated rats were returned to the mother. After 24 h and 14 days, fixed brain sections revealed wide-spread fluorescence in elongated or pyramidal-shaped cell profiles in a discrete internal cortical layer, consistent with layer V pyramidal cells. Labeled neurons displayed spontaneous synaptic activity using the slice patch clamp method. These results suggest that these dyes are effective tools for pathophysiological and slice patch clamp studies focused on specific neuron groups.

本文言語English
ページ(範囲)183-188
ページ数6
ジャーナルBrain Research Protocols
13
3
DOI
出版ステータスPublished - 2004 8

ASJC Scopus subject areas

  • Neuroscience(all)

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