Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells

Tetsutaro Sumiyoshi, Kaoru Sato, Hitomi Yamamoto, Yuka W. Iwasaki, Haruhiko Siomi, Mikiko C. Siomi

研究成果: Article

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In Drosophila germ cells, PIWI-interacting RNAs (piR-NAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical analyses has not been available. Here, we show that CRISPR-mediated loss of function of lethal (3) malignant brain tumor [l(3)mbt] leads to ectopic activation of the germ-specific pingpong cycle in ovarian somatic cells. Perinuclear foci resembling nuage, the ping-pong center, appeared following l(3)mbt mutation. This activation of the ping-pong machinery in cultured cells will greatly facilitate elucidation of the mechanism underlying secondary piRNA biogenesis in Drosophila.

元の言語English
ページ(範囲)1617-1622
ページ数6
ジャーナルGenes and Development
30
発行部数14
DOI
出版物ステータスPublished - 2016 7 15

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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