@article{492d40eadb4d463fb5bb5407469a55f4,
title = "Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells",
abstract = "Resolving the immunogenicity of cells derived from induced pluripotent stem cells (iPSCs) remains an important challenge for cell transplant strategies that use banked allogeneic cells. Thus, we evaluated the immunogenicity of mouse fetal neural stem/progenitor cells (fetus-NSPCs) and iPSC-derived neural stem/progenitor cells (iPSC-NSPCs) both in vitro and in vivo. Flow cytometry revealed the low expression of immunological surface antigens, and these cells survived in all mice when transplanted syngeneically into subcutaneous tissue and the spinal cord. In contrast, an allogeneic transplantation into subcutaneous tissue was rejected in all mice, and allogeneic cells transplanted into intact and injured spinal cords survived for 3 months in approximately 20% of mice. In addition, cell survival was increased after co-treatment with an immunosuppressive agent. Thus, the immunogenicity and post-transplantation immunological dynamics of iPSC-NSPCs resemble those of fetus-NSPCs.",
author = "Go Itakura and Masahiro Ozaki and Narihito Nagoshi and Soya Kawabata and Yuichiro Nishiyama and Keiko Sugai and Tsuyoshi Iida and Rei Kashiwagi and Toshiki Ookubo and Kaori Yastake and Kohei Matsubayashi and Jun Kohyama and Akio Iwanami and Morio Matsumoto and Masaya Nakamura and Hideyuki Okano",
note = "Funding Information: We thank Dr. Ryoko Araki (Transcriptome Research Group, National Institute of Radiological Sciences) for the mouse iPSC clone 2A3F, 2A4F, Prof. Yutaka Kawakami (Keio University), Prof. Akihiko Yoshimura (Keio University) for invaluable comments for the study design and Prof. Douglas Sipp (Keio University) for invaluable comments for the manuscript. We are also grateful for K. Fukushima, A. Kohzuki, R. Yamaguchi, M. Isoda, F. Renault-Mihara, S. Shibata, M. Shinozaki, T. Konomi, Y. Kobayashi, M. Takano, S. Nishimura, S. Tashiro, K. Kojima, S. Ito, Y. Tanimoto, Y. Hoshino and T. Harada, who are members of the spinal cord research team in the Department of Orthopaedic Surgery, Physiology and Rehabilitation Medicine, Keio University School of Medicine. This work was supported by the Research Center Network for Realization of Regenerative Medicine by the Japan Science and Technology Agency (JST) and the Japan Agency for Medical Research and Development (AMED) (H.O. and M.N.), Grant-in-Aid for Scientific Research by Japan Society for the Promotion of Science (A.I.) and grant by The General Insurance Association of Japan (A.I.). H.O. is a paid Scientific Advisory Board member for SanBio Co., Ltd and K Pharma Inc. M.N. is a paid Scientific Advisory Board member for K Pharma Inc. Publisher Copyright: {\textcopyright} 2017 The Author(s).",
year = "2017",
month = dec,
day = "1",
doi = "10.1038/s41598-017-13522-w",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}