Luminol-dependent photoemission from single neutrophil stimulated by phorbol ester and calcium ionophore - role of degranulation and myeloperoxidase

Makoto Suematsu, Chikara Oshio, Soichiro Miura, Masayuki Suzuki, Shigenari Houzawa, Masaharu Tsuchiya

研究成果: Article査読

35 被引用数 (Scopus)

抄録

Luminol-dependent photonic burst from phorbol ester-treated single neutrophil was visually investigated by using an ultrasensitive photonic image intensifier microscope. Neutrophils stimulated by phorbol myristate acetate (0.1 μg/ml) alone produced a negligible level of photonic activities in the presence of luminol (10 μg/ml). The additional application of 0.1 μM Ca2+ ionophore A23187 induced explosive changes of photonic burst corresponding to the distribution of neutrophils, and these photonic activities were gradually spread to extracellular space. Sodium azide, which prevents myeloperoxidase activity, inhibited Ca2+ ionophore-induced photonic burst from phorbol ester-treated neutrophil. These findings suggest a prerequisite role of degranulation and myeloperoxidase release in luminol-dependent photoemission from stimulated neutrophils.

本文言語English
ページ(範囲)106-111
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
155
1
DOI
出版ステータスPublished - 1988 8 30

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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