Lymphatic marker podoplanin/D2-40 in human advanced cirrhotic liver- Re-evaluations of microlymphatic abnormalities

Hiroaki Yokomori, Masaya Oda, Fumihiko Kaneko, Shigeyuki Kawachi, Minoru Tanabe, Kazunori Yoshimura, Yuukou Kitagawa, Toshifumi Hibi

研究成果: Article

11 引用 (Scopus)

抄録

Background: From the morphological appearance, it was impossible to distinguish terminal portal venules from small lymphatic vessels in the portal tract even using histochemical microscopic techniques. Recently, D2-40 was found to be expressed at a high level in lymphatic endothelial cells (LECs). This study was undertaken to elucidate hepatic lymphatic vessels during progression of cirrhosis by examining the expression of D2-40 in LECs.Methods: Surgical wedge biopsy specimens were obtained from non-cirrhotic portions of human livers (normal control) and from cirrhotic livers (LC) (Child A-LC and Child C-LC). Immunohistochemical (IHC), Western blot, and immunoelectron microscopic studies were conducted using D2-40 as markers for lymphatic vessels, as well as CD34 for capillary blood vessels.Results: Imunostaining of D2-40 produced a strong reaction in lymphatic vessels only, especially in Child C-LC. It was possible to distinguish the portal venules from the small lymphatic vessels using D-40. Immunoelectron microscopy revealed strong D2-40 expression along the luminal and abluminal portions of the cell membrane of LECs in Child C-LC tissue.Conclusion: It is possible to distinguish portal venules from small lymphatic vessels using D2-40 as marker. D2-40- labeling in lymphatic capillary endothelial cells is related to the degree of fibrosis in cirrhotic liver.

元の言語English
記事番号131
ジャーナルBMC Gastroenterology
10
DOI
出版物ステータスPublished - 2010 11 8

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Lymphatic Vessels
Liver
Endothelial Cells
Venules
Fibrosis
Immunoelectron Microscopy
Blood Vessels
Western Blotting
Cell Membrane
Biopsy

ASJC Scopus subject areas

  • Gastroenterology

これを引用

Lymphatic marker podoplanin/D2-40 in human advanced cirrhotic liver- Re-evaluations of microlymphatic abnormalities. / Yokomori, Hiroaki; Oda, Masaya; Kaneko, Fumihiko; Kawachi, Shigeyuki; Tanabe, Minoru; Yoshimura, Kazunori; Kitagawa, Yuukou; Hibi, Toshifumi.

:: BMC Gastroenterology, 巻 10, 131, 08.11.2010.

研究成果: Article

Yokomori, Hiroaki ; Oda, Masaya ; Kaneko, Fumihiko ; Kawachi, Shigeyuki ; Tanabe, Minoru ; Yoshimura, Kazunori ; Kitagawa, Yuukou ; Hibi, Toshifumi. / Lymphatic marker podoplanin/D2-40 in human advanced cirrhotic liver- Re-evaluations of microlymphatic abnormalities. :: BMC Gastroenterology. 2010 ; 巻 10.
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abstract = "Background: From the morphological appearance, it was impossible to distinguish terminal portal venules from small lymphatic vessels in the portal tract even using histochemical microscopic techniques. Recently, D2-40 was found to be expressed at a high level in lymphatic endothelial cells (LECs). This study was undertaken to elucidate hepatic lymphatic vessels during progression of cirrhosis by examining the expression of D2-40 in LECs.Methods: Surgical wedge biopsy specimens were obtained from non-cirrhotic portions of human livers (normal control) and from cirrhotic livers (LC) (Child A-LC and Child C-LC). Immunohistochemical (IHC), Western blot, and immunoelectron microscopic studies were conducted using D2-40 as markers for lymphatic vessels, as well as CD34 for capillary blood vessels.Results: Imunostaining of D2-40 produced a strong reaction in lymphatic vessels only, especially in Child C-LC. It was possible to distinguish the portal venules from the small lymphatic vessels using D-40. Immunoelectron microscopy revealed strong D2-40 expression along the luminal and abluminal portions of the cell membrane of LECs in Child C-LC tissue.Conclusion: It is possible to distinguish portal venules from small lymphatic vessels using D2-40 as marker. D2-40- labeling in lymphatic capillary endothelial cells is related to the degree of fibrosis in cirrhotic liver.",
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AU - Oda, Masaya

AU - Kaneko, Fumihiko

AU - Kawachi, Shigeyuki

AU - Tanabe, Minoru

AU - Yoshimura, Kazunori

AU - Kitagawa, Yuukou

AU - Hibi, Toshifumi

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