Magnetic enzymatic platform for organophosphate pesticide detection using boron-doped diamond electrodes

Flavio Pino, Tribidasari A. Ivandini, Kazuya Nakata, Akira Fujishima, Arben Merkoçi, Yasuaki Einaga

研究成果: Article

8 引用 (Scopus)

抜粋

A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10 -9 to 10 -5 M. The estimated limits of detection based on IC 10 (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10 -10 M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.

元の言語English
ページ(範囲)1061-1068
ページ数8
ジャーナルanalytical sciences
31
発行部数10
DOI
出版物ステータスPublished - 2015

ASJC Scopus subject areas

  • Analytical Chemistry

フィンガープリント Magnetic enzymatic platform for organophosphate pesticide detection using boron-doped diamond electrodes' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用