Major role of cytochrome P450 2E1 in ethanol oxidation by Kupffer cells

Yuji Nakamura, Hirokazu Yokoyama, Shinzo Kato, Hiromasa Ishii

研究成果: Article

3 引用 (Scopus)

抄録

To examine ethanol oxidation by cultured rat Kupffer cells, we measured [14C]-acetate formation from [14C]-ethanol. Diallyl sulfide (1 mM), and miconazole (60 μ M), respectively inhibitors of cytochrome P450 2E1 and NADPH cytochrome P450 reductase, significantly attenuated acetate formation from 20 mM or 50 mM ethanol. Sodium azide (1 mM), an inhibitor of catalase, significantly suppressed acetate formation from 50 mM ethanol and tended to suppress it at 20 mM. However, 4-methylpyrazole (2 mM), an inhibitor of class I alcohol dehydrogenase, failed to suppress ethanol oxidation at either concentration. A reverse-transcription polymerase chain reaction demonstrated that Kupffer cells carry mRNAs for cytochrome P450 2E1 and catalase, but not for class I alcohol dehydrogenase. It can thus be argued that cytochrome P450 2E1 plays a major role in ethanol oxidation by Kupffer cells.

元の言語English
ページ(範囲)15-25
ページ数11
ジャーナルResearch Communications in Alcohol and Substances of Abuse
20
発行部数1-2
出版物ステータスPublished - 1999

Fingerprint

Cytochrome P-450 CYP2E1
Kupffer Cells
Ethanol
Oxidation
Acetates
Alcohol Dehydrogenase
Catalase
Miconazole
NADPH-Ferrihemoprotein Reductase
Sodium Azide
Polymerase chain reaction
Transcription
Reverse Transcription
Rats
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

これを引用

@article{0b0094645b0f4bfe8359e2bf8833ec4f,
title = "Major role of cytochrome P450 2E1 in ethanol oxidation by Kupffer cells",
abstract = "To examine ethanol oxidation by cultured rat Kupffer cells, we measured [14C]-acetate formation from [14C]-ethanol. Diallyl sulfide (1 mM), and miconazole (60 μ M), respectively inhibitors of cytochrome P450 2E1 and NADPH cytochrome P450 reductase, significantly attenuated acetate formation from 20 mM or 50 mM ethanol. Sodium azide (1 mM), an inhibitor of catalase, significantly suppressed acetate formation from 50 mM ethanol and tended to suppress it at 20 mM. However, 4-methylpyrazole (2 mM), an inhibitor of class I alcohol dehydrogenase, failed to suppress ethanol oxidation at either concentration. A reverse-transcription polymerase chain reaction demonstrated that Kupffer cells carry mRNAs for cytochrome P450 2E1 and catalase, but not for class I alcohol dehydrogenase. It can thus be argued that cytochrome P450 2E1 plays a major role in ethanol oxidation by Kupffer cells.",
author = "Yuji Nakamura and Hirokazu Yokoyama and Shinzo Kato and Hiromasa Ishii",
year = "1999",
language = "English",
volume = "20",
pages = "15--25",
journal = "Research Communications in Alcohol and Substances of Abuse",
issn = "1080-8388",
publisher = "PJD Publications Ltd",
number = "1-2",

}

TY - JOUR

T1 - Major role of cytochrome P450 2E1 in ethanol oxidation by Kupffer cells

AU - Nakamura, Yuji

AU - Yokoyama, Hirokazu

AU - Kato, Shinzo

AU - Ishii, Hiromasa

PY - 1999

Y1 - 1999

N2 - To examine ethanol oxidation by cultured rat Kupffer cells, we measured [14C]-acetate formation from [14C]-ethanol. Diallyl sulfide (1 mM), and miconazole (60 μ M), respectively inhibitors of cytochrome P450 2E1 and NADPH cytochrome P450 reductase, significantly attenuated acetate formation from 20 mM or 50 mM ethanol. Sodium azide (1 mM), an inhibitor of catalase, significantly suppressed acetate formation from 50 mM ethanol and tended to suppress it at 20 mM. However, 4-methylpyrazole (2 mM), an inhibitor of class I alcohol dehydrogenase, failed to suppress ethanol oxidation at either concentration. A reverse-transcription polymerase chain reaction demonstrated that Kupffer cells carry mRNAs for cytochrome P450 2E1 and catalase, but not for class I alcohol dehydrogenase. It can thus be argued that cytochrome P450 2E1 plays a major role in ethanol oxidation by Kupffer cells.

AB - To examine ethanol oxidation by cultured rat Kupffer cells, we measured [14C]-acetate formation from [14C]-ethanol. Diallyl sulfide (1 mM), and miconazole (60 μ M), respectively inhibitors of cytochrome P450 2E1 and NADPH cytochrome P450 reductase, significantly attenuated acetate formation from 20 mM or 50 mM ethanol. Sodium azide (1 mM), an inhibitor of catalase, significantly suppressed acetate formation from 50 mM ethanol and tended to suppress it at 20 mM. However, 4-methylpyrazole (2 mM), an inhibitor of class I alcohol dehydrogenase, failed to suppress ethanol oxidation at either concentration. A reverse-transcription polymerase chain reaction demonstrated that Kupffer cells carry mRNAs for cytochrome P450 2E1 and catalase, but not for class I alcohol dehydrogenase. It can thus be argued that cytochrome P450 2E1 plays a major role in ethanol oxidation by Kupffer cells.

UR - http://www.scopus.com/inward/record.url?scp=0033404218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033404218&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0033404218

VL - 20

SP - 15

EP - 25

JO - Research Communications in Alcohol and Substances of Abuse

JF - Research Communications in Alcohol and Substances of Abuse

SN - 1080-8388

IS - 1-2

ER -