Mammalian actin binding protein 1 is essential for endocytosis but not lamellipodia formation: Functional analysis by RNA interference

Setsuko Mise-Omata, Benjamin Montagne, Marcel Deckert, Jürgen Wienands, Oreste Acuto

研究成果: Article査読

45 被引用数 (Scopus)

抄録

Mammalian actin binding protein 1 (mAbp1, also called SH3P7/Hip55) is structurally and functionally related to yeast Abp1 and to cortactin, both of which have been implicated in endocytotic processes. mAbp1 associates through its SH3 domain with dynamin, a large GTPase essential for vesicle fission. To clarify the function of mAbp1, we specifically knocked down its expression in human embryonic kidney 293T cells, using RNA interference (RNAi). Co-transfection of a short interfering RNA (siRNA) together with a plasmid coding for a surface marker, followed by purification of transfected cells, enabled us to obtain a cell population having up to 90% inhibition of mAbp1 expression. In mAbp1-knocked down cells, transferrin (Tf) receptor endocytosis was significantly inhibited and intracellular distribution of the early endosomal compartment was modified. In contrast, in these cells actin and microtubule filaments appeared normal, and formation of lamellipodia induced by active Rac was not inhibited. This study provides definitive evidence that mAbp1 is indispensable for receptor-mediated endocytosis.

本文言語English
ページ(範囲)704-710
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
301
3
DOI
出版ステータスPublished - 2003 2月 14
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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