Mass imaging and identification of biomolecules with MALDI-QIT-TOF-based system

Shuichi Shimma, Yuki Sugiura, Takahiro Hayasaka, Nobuhiro Zaima, Mineo Matsumoto, Mitsutoshi Setou

研究成果: Article査読

132 被引用数 (Scopus)

抄録

Imaging mass spectrometry is becoming a popular visualization technique in the medical and biological sciences. For its continued development, the ability to both visualize and identify molecules directiy on the tissue surface using tandem mass spectrometry (MSn) is essential. We established an imaging system based on a matrix-assisted laser/desorption ionization quadrupole ion trap time-of-flight type instrument (AXIMA-QIT, Shimadzu, Kyoto, Japan), which was compatible with both imaging and highly sensitive MSn. In this paper, we present the operating conditions of the AXIMA-QIT as an imaging instrument and introduce the data converter we developed that is available free of charge. The converted data can be applied to Biomap, the commonly used visualization software. For the feasibility experiments, we demonstrated the visualization of phospholipids, glycolipid, and tryptic-digested proteins in the mouse cerebellum. The visualized lipids were successfully identified by MS n directiy on the tissue surface, with a strong ability to isolate precursor ions. In the analysis of tryptic-digested proteins, we compared the product ion spectra between AXIMA-QIT and a tandem TOF-type instrument The results confirmed that AXIMA-QIT can provide a high quality of product ion spectra even on the tissue surface.

本文言語English
ページ(範囲)878-885
ページ数8
ジャーナルAnalytical chemistry
80
3
DOI
出版ステータスPublished - 2008 2月 1
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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