Measurement for optical properties of mitochondria in vitro

Akio Nakajima, Miya Yoshikawa, Tsunenori Arai, Yuji M.D. Morimoto, Makoto Kikuchi, et.al.

研究成果: Conference contribution

4 被引用数 (Scopus)

抄録

The effectiveness of low-intensity light for clinical therapies is well demonstrated, however, the principles of these therapies are not well understood. Our aim of this study is the investigation of optical properties of mitochondria because it seems to be the most important cellular organella in terms of the light-cell interaction. We measured the optical absorption characteristics of an isolated mitochondrial suspension in the range of 300 to 900 nm by means of the transmission methods with an integral sphere. Mitochondrial specific absorption coefficients (ml/g/cm-1) were carried out by the transmission spectrum. The specific absorption coefficient at the maximum absorption of cytochrome cγ peaked about 560 ml/g/cm-1 at 418 nm. Many papers described that the effect of the low intensity light to the biological tissue would be drastically changed by the irradiation conditions. We could calculate a deposit energy of irradiated light in a mitochondrion from our results. We think our results may be useful to describe the quantitative explanation in energy balance for the interaction between the light and mitochondria.

本文言語English
ホスト出版物のタイトルProceedings of SPIE - The International Society for Optical Engineering
出版社Publ by Society of Photo-Optical Instrumentation Engineers
ページ62-67
ページ数6
ISBN(印刷版)0819411108
出版ステータスPublished - 1993
イベントLow-Energy Laser Effects on Biological Systems - Los Angeles, CA, USA
継続期間: 1993 1月 191993 1月 20

出版物シリーズ

名前Proceedings of SPIE - The International Society for Optical Engineering
1883
ISSN(印刷版)0277-786X

Other

OtherLow-Energy Laser Effects on Biological Systems
CityLos Angeles, CA, USA
Period93/1/1993/1/20

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • コンピュータ サイエンスの応用
  • 応用数学
  • 電子工学および電気工学

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