Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent

Takamitsu Nakano, Yoshiharu Tokita, Takeaki Nagamine, Akira Tanaka, Mitsuyo Okazaki, Shinichi Yatsuzuka, Noriko Tamei, Younosuke Shimomura, Yoshitaka Ando, Suguru Akamatsu, Masaki Q. Fujita, Katsuyuki Nakajima

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Background: Serum concentration of remnant-like lipoprotein particles (RLP) have been measured by cholesterol as RLP-C for clinical diagnostic purpose. However, the measurement of TG in RLP and the ratio of RLP-TG/total TG has not been well established. Method: Highly sensitive triglyceride assay reagent (TG-EX) was used for RLP-TG assay and compared with the previously used TG reagent (Determiner LTGII). Sera in health check-up populations, cardiovascular disease, diabetes and oral fat load cases were used for the evaluation of the new RLP-TG assay. Serum TC, TG, HDL-C, LDL-C and RLP-C concentrations were also determined in above cases. Results: The detection limit of new RLP-TG using TG-EX was 2.0. mg/dl. The within-run imprecision (n = 10) was CV = 3.0% (RLP-TG: 4.1. mg. ±. 0.7. mg/dl), CV = 1.4% (RLP-TG: 42.0. ±. 0.6. mg/dl) and CV = 0.5% (RLP-TG: 100.6. ±. 0.6. mg/dl). Cut-off value (75 percentile) of RLP-TG determined in the fasting Japanese population was 13.1. mg/dl in men and 9.9. mg/dl in women. In patients with metabolic syndrome, cardiovascular disease and diabetes, RLP-TG levels were significantly higher than those in normal control subjects. RLP-TG levels increased significantly after an oral fat load and the ratio of RLP-TG/total TG increased. >. 3-fold compared to the ratio in the fasting state. Approximately 80% of TG increased after an oral fat load was TG derived from remnant lipoproteins. Conclusion: Normal range of plasma RLP-TG in the fasting Japanese population was first determined using a highly sensitive TG assay reagent. RLP-TG was shown to be higher in cases with metabolic syndrome, cardiovascular disease, etc and a better marker than RLP-C for the measurement of postprandial remnant lipoproteins, together with total TG for RLP-TG/total TG ratio.

元の言語English
ページ(範囲)71-78
ページ数8
ジャーナルClinica Chimica Acta
412
発行部数1-2
DOI
出版物ステータスPublished - 2011 1 14

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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    Nakano, T., Tokita, Y., Nagamine, T., Tanaka, A., Okazaki, M., Yatsuzuka, S., Tamei, N., Shimomura, Y., Ando, Y., Akamatsu, S., Fujita, M. Q., & Nakajima, K. (2011). Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent. Clinica Chimica Acta, 412(1-2), 71-78. https://doi.org/10.1016/j.cca.2010.09.040