Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy

Keisuke Isobe, Hiroshi Hashimoto, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

研究成果: Article査読

6 被引用数 (Scopus)

抄録

We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form.

本文言語English
ページ(範囲)687-693
ページ数7
ジャーナルBiomedical Optics Express
1
2
DOI
出版ステータスPublished - 2010 9月 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 原子分子物理学および光学

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