Mechanical cell contact system by a parylene rail filter for study of cell-cell interaction mediated by connexin gap junction

Y. Abe, K. Kamiya, T. Osaki, R. Kawano, K. Akiyoshi, N. Miki, S. Takeuchi

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

This paper proposes a mechanical cell contact system using a parylene rail filter (PRF) for in vitro analysis of connexin functions in cell-cell interaction. Connexin is a membrane protein that mediates interaction between contacting cells and allows small molecules to migrate between the cells. To study this molecular transfer and connexin function, cells must be maintained contact throughout experiments for days under the microscope. We reported a mechanical single liposome trap system with various advantages in MEMS2013 [1]. In this present study, we developed a mechanical cell trap system that traps cells in contact with each other by using a micropatterned parylene rail. The number of trapped cells could be controlled by the length of the rail and the calcein dye transfer between the neighboring cells via connexin gap junctions (GJs) was successfully observed.

本文言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版社Chemical and Biological Microsystems Society
ページ407-409
ページ数3
ISBN(印刷版)9781632666246
出版ステータスPublished - 2013
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
継続期間: 2013 10 272013 10 31

出版物シリーズ

名前17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

ASJC Scopus subject areas

  • Bioengineering

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