Mechanical regulation of oligodendrocyte morphology and maturation by the mechanosensor p130Cas

Takeshi Shimizu, Yasuyuki Osanai, Kenji F. Tanaka, Truc Quynh Thai, Manabu Abe, Rie Natsume, Kenji Sakimura, Kazuhiro Ikenaka

研究成果: Article

抜粋

Oligodendrocytes (OLs) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimulation can be transduced into intracellular biochemical signals through mechanosensors, such as integrin, p130Cas, talin and vinculin. However, the molecular mechanisms underlying the mechanical regulation of OLs by mechanosensors remain largely unknown. We found that morphology of OL was affected by knockdown of the mechanosensors p130Cas or talin1. Stretching of OL precursor cells induced the phosphorylation of p130Cas and talin-associated assembly of vinculin. Shear stress decreased the number of OL processes, whereas these effects were mechanically suppressed by dominant-negative (DN) p130Cas, but not by DN-talin1. To investigate the roles of p130Cas in post-natal OLs in vivo, we constructed a novel p130Cas knock-in mouse and found overexpression of p130Cas in vivo affected the number of mature OLs in the cortex. These results indicate that the mechanosensor p130Cas controls both OL morphogenesis and maturation. (Figure presented.).

元の言語English
ページ(範囲)158-172
ページ数15
ジャーナルJournal of Neurochemistry
150
発行部数2
DOI
出版物ステータスPublished - 2019 7

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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  • これを引用

    Shimizu, T., Osanai, Y., Tanaka, K. F., Thai, T. Q., Abe, M., Natsume, R., Sakimura, K., & Ikenaka, K. (2019). Mechanical regulation of oligodendrocyte morphology and maturation by the mechanosensor p130Cas. Journal of Neurochemistry, 150(2), 158-172. https://doi.org/10.1111/jnc.14657