Mechanism to preserve phrenic nerve function during photosensitization reaction: Drug uptake and photosensitization reaction effect on electric propagation

Haruka Takahashi, Risa Hamada, Emiyu Ogawa, Tsunenori Arai

研究成果: Conference contribution

抜粋

To study a mechanism of phrenic nerve preservation phenomena during a photosensitization reaction, we investigated an uptake of talaporfin sodium and photosensitization reaction effect on an electric propagation. Right phrenic nerve was completely preserved after superior vena cava isolations using the photosensitization reaction in canine animal experiments, in spite of adjacent myocardium was electrically blocked. We predicted that low drug uptake and/or low photosensitization reaction effect on the nerve might be a mechanism of that phenomena. To investigate uptake to various nerve tissue, a healthy extracted crayfish ventral nerve cord and an extracted porcine phrenic nerve were immersed in 20 μg/ml talaporfin sodium solution for 0-240 min. The mean talaporfin sodium fluorescence brightness increased depending on the immersion time. This brightness saturated around the immersion time of 120 min. We found that talaporfin sodium uptake inside the perineurium which directly related to the electric propagation function was lower than that of outside in the porcine phrenic nerve. To investigate photosensitization reaction effect on electric propagation, the crayfish nerve was immersed into the same solution for 15 min and irradiated by a 663 nm laser light with 120 mW/cm2. Since we found the action potential disappeared when the irradiation time was 25-65 s, we consider that the crayfish nerve does not tolerant to the photosensitization reaction on electric propagation function at atmospheric pressure. From these results, we think that the low uptake of talaporfin sodium inside the perineurium and low oxygen partial pressure of nerve might be the possible mechanism to preserve phrenic nerve in vivo.

元の言語English
ホスト出版物のタイトルOptical Methods for Tumor Treatment and Detection
ホスト出版物のサブタイトルMechanisms and Techniques in Photodynamic Therapy XXVII
出版者SPIE
10476
ISBN(電子版)9781510614376
DOI
出版物ステータスPublished - 2018 1 1
イベントOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVII 2018 - San Francisco, United States
継続期間: 2018 1 272018 1 29

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVII 2018
United States
San Francisco
期間18/1/2718/1/29

    フィンガープリント

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

これを引用

Takahashi, H., Hamada, R., Ogawa, E., & Arai, T. (2018). Mechanism to preserve phrenic nerve function during photosensitization reaction: Drug uptake and photosensitization reaction effect on electric propagation. : Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVII (巻 10476). [1047610] SPIE. https://doi.org/10.1117/12.2289200