Mechanosensitive cation channels mediate afferent arteriolar myogenic constriction in the isolated rat kidney

Tsuneo Takenaka, Hiromichi Suzuki, Hirokazu Okada, Koichi Hayashi, Yoshihiko Kanno, Takao Saruta

研究成果: Article査読

41 被引用数 (Scopus)

抄録

1. In order to assess ionic mechanisms mediating renal afferent arteriolar myogenic constriction, experiments were performed using isolated perfused hydronephrotic rat kidneys. 2. Increasing pressure progressively constricted the afferent arteriole (-0.26 ± 0.02% mmHg-1, n = 21, r = 0.97). Gadolinium (10 μM), a mechanosensitive cation channel blocker, abolished this myogenic constriction. However, high potassium media (30 mM) constricted the afferent arteriole in the presence of gadolinium. 3. Lowering extracellular sodium concentration gradually attenuated afferent arteriolar myogenic constriction. In the perfusate containing 50 mM sodium, the myogenic response was arrested. 4. Afferent arteriolar myogenic constriction was prevented in calcium-free perfusate or by the L-type calcium channel blocker diltiazem (10 μM). 5. Our present findings provide evidence that increasing pressure gates mechanosensitive cation channels on the afferent arteriole, thereby eliciting membrane depolarization and activating voltage-dependent calcium channels.

本文言語English
ページ(範囲)245-253
ページ数9
ジャーナルJournal of Physiology
511
1
DOI
出版ステータスPublished - 1998 8 15

ASJC Scopus subject areas

  • 生理学

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