Melanocyte lineage-specific antigen gp100 is recognized by melanoma-derived tumor-infiltrating lymphocytes

Alexander B H Bakker, Marco W J Schreurs, Annemiek J. De Boer, Yutaka Kawakami, Steven A. Rosenberg, Gosse J. Adema, Carl G. Figdor

研究成果: Article

501 引用 (Scopus)

抄録

We recently isolated a cDNA clone that encodes the melanocyte lineage-specific antigen glycoprotein (gp)100. Antibodies directed against gp100 are an important tool in the diagnosis of human melanoma. Since the gp100 antigen is highly expressed in melanocytic cells, we investigated whether this antigen might serve as a target for antimelanoma cytotoxic T lymphocytes (CTL). Here, we demonstrate that cytotoxic tumor-infiltrating lymphocytes (TIL) derived from a melanoma patient (TIL 1200) are directed against gp100. HLA-A2.1+ melanoma cells are lysed by TIL from this patient. In addition, murine double transfectants, expressing both HLA-A2.1 and gp100, are lysed by TIL 1200, whereas transfectants expressing only HLA-A2.1 are not susceptible to lysis. Furthermore, the HLA-A2.1+ melanoma cell line BLM, which lacks gp100 expression and is resistant to lysis, becomes susceptible after transfection of gp100 cDNA. Finally, HLA-A2.1+ normal melanocytes are lysed by TIL 1200. These data demonstrate that the melanocyte differentiation antigen gp100 can be recognized in the context of HLA-A2.1 by CTL from a melanoma patient. Gp100 may therefore constitute a useful target for specific immunotherapy against melanoma, provided that no unacceptable cytotoxicity towards normal tissue is observed.

元の言語English
ページ(範囲)1005-1009
ページ数5
ジャーナルJournal of Experimental Medicine
179
発行部数3
出版物ステータスPublished - 1994 3 1
外部発表Yes

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gp100 Melanoma Antigen
Tumor-Infiltrating Lymphocytes
Melanoma
Melanocytes
Cytotoxic T-Lymphocytes
Antigens
Complementary DNA
Immunotherapy
Transfection
Glycoproteins
Clone Cells
Cell Line
Antibodies

ASJC Scopus subject areas

  • Immunology

これを引用

Bakker, A. B. H., Schreurs, M. W. J., De Boer, A. J., Kawakami, Y., Rosenberg, S. A., Adema, G. J., & Figdor, C. G. (1994). Melanocyte lineage-specific antigen gp100 is recognized by melanoma-derived tumor-infiltrating lymphocytes. Journal of Experimental Medicine, 179(3), 1005-1009.

Melanocyte lineage-specific antigen gp100 is recognized by melanoma-derived tumor-infiltrating lymphocytes. / Bakker, Alexander B H; Schreurs, Marco W J; De Boer, Annemiek J.; Kawakami, Yutaka; Rosenberg, Steven A.; Adema, Gosse J.; Figdor, Carl G.

:: Journal of Experimental Medicine, 巻 179, 番号 3, 01.03.1994, p. 1005-1009.

研究成果: Article

Bakker, ABH, Schreurs, MWJ, De Boer, AJ, Kawakami, Y, Rosenberg, SA, Adema, GJ & Figdor, CG 1994, 'Melanocyte lineage-specific antigen gp100 is recognized by melanoma-derived tumor-infiltrating lymphocytes', Journal of Experimental Medicine, 巻. 179, 番号 3, pp. 1005-1009.
Bakker ABH, Schreurs MWJ, De Boer AJ, Kawakami Y, Rosenberg SA, Adema GJ その他. Melanocyte lineage-specific antigen gp100 is recognized by melanoma-derived tumor-infiltrating lymphocytes. Journal of Experimental Medicine. 1994 3 1;179(3):1005-1009.
Bakker, Alexander B H ; Schreurs, Marco W J ; De Boer, Annemiek J. ; Kawakami, Yutaka ; Rosenberg, Steven A. ; Adema, Gosse J. ; Figdor, Carl G. / Melanocyte lineage-specific antigen gp100 is recognized by melanoma-derived tumor-infiltrating lymphocytes. :: Journal of Experimental Medicine. 1994 ; 巻 179, 番号 3. pp. 1005-1009.
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abstract = "We recently isolated a cDNA clone that encodes the melanocyte lineage-specific antigen glycoprotein (gp)100. Antibodies directed against gp100 are an important tool in the diagnosis of human melanoma. Since the gp100 antigen is highly expressed in melanocytic cells, we investigated whether this antigen might serve as a target for antimelanoma cytotoxic T lymphocytes (CTL). Here, we demonstrate that cytotoxic tumor-infiltrating lymphocytes (TIL) derived from a melanoma patient (TIL 1200) are directed against gp100. HLA-A2.1+ melanoma cells are lysed by TIL from this patient. In addition, murine double transfectants, expressing both HLA-A2.1 and gp100, are lysed by TIL 1200, whereas transfectants expressing only HLA-A2.1 are not susceptible to lysis. Furthermore, the HLA-A2.1+ melanoma cell line BLM, which lacks gp100 expression and is resistant to lysis, becomes susceptible after transfection of gp100 cDNA. Finally, HLA-A2.1+ normal melanocytes are lysed by TIL 1200. These data demonstrate that the melanocyte differentiation antigen gp100 can be recognized in the context of HLA-A2.1 by CTL from a melanoma patient. Gp100 may therefore constitute a useful target for specific immunotherapy against melanoma, provided that no unacceptable cytotoxicity towards normal tissue is observed.",
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AU - Bakker, Alexander B H

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AU - De Boer, Annemiek J.

AU - Kawakami, Yutaka

AU - Rosenberg, Steven A.

AU - Adema, Gosse J.

AU - Figdor, Carl G.

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N2 - We recently isolated a cDNA clone that encodes the melanocyte lineage-specific antigen glycoprotein (gp)100. Antibodies directed against gp100 are an important tool in the diagnosis of human melanoma. Since the gp100 antigen is highly expressed in melanocytic cells, we investigated whether this antigen might serve as a target for antimelanoma cytotoxic T lymphocytes (CTL). Here, we demonstrate that cytotoxic tumor-infiltrating lymphocytes (TIL) derived from a melanoma patient (TIL 1200) are directed against gp100. HLA-A2.1+ melanoma cells are lysed by TIL from this patient. In addition, murine double transfectants, expressing both HLA-A2.1 and gp100, are lysed by TIL 1200, whereas transfectants expressing only HLA-A2.1 are not susceptible to lysis. Furthermore, the HLA-A2.1+ melanoma cell line BLM, which lacks gp100 expression and is resistant to lysis, becomes susceptible after transfection of gp100 cDNA. Finally, HLA-A2.1+ normal melanocytes are lysed by TIL 1200. These data demonstrate that the melanocyte differentiation antigen gp100 can be recognized in the context of HLA-A2.1 by CTL from a melanoma patient. Gp100 may therefore constitute a useful target for specific immunotherapy against melanoma, provided that no unacceptable cytotoxicity towards normal tissue is observed.

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