Melanoma tumor-infiltrating lymphocytes derived from four distinct anatomic sites obtained from a single patient: Comparison of functional reactivity and melanoma antigen recognition

John R. Yannelli, Susan McConnell, Linda Parker, Michael Nishimura, Paul Robbins, James Yang, Mona El Gamil, Yutaka Kawakami

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Tumor-infiltrating lymphocytes (TILs) were grown from four distinct anatomic sites from a patient with metastatic melanoma. The metastatic sites included a tumor-involved lymph node, a subcutaneous lesion obtained from the chest wall, a portion of bowel, and adrenal gland. TILs grown from each anatomic site over the course of 20 days in the presence of 6,000 IU/ml recombinant interleukin-2 exhibited comparable growth rates. Between days 30 and 45, the TILs were a mixture of CD3+CD4+and CD3+CD8+lymphocytes expressing the αβ form of the T-cell receptor. TILs derived from each anatomic site specifically lysed autologous tumor obtained from all four anatomic sites. In fine specificity analysis, the TILs exhibited human leukocyte antigen (HLA-A2)-restricted lysis of fresh tumor targets and cultured melanoma cell lines. Each TIL recognized a product of the MART-1 gene, and specifically, the monomer peptide MART-127–35. Thus lymphocytes reactive with the MART-1 melanoma antigen appeared to be widely distributed in diverse metastases in this patient. This information, along with previous data on the reactivity of multiple patients to this antigen, attests to its dominance in the immune reactivity of humans to melanoma.

本文言語English
ページ(範囲)263-271
ページ数9
ジャーナルJournal of Immunotherapy
18
4
DOI
出版ステータスPublished - 1995 11月

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学
  • 薬理学
  • 癌研究

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