Membrane potential measurement of protoplasts isolated from vigna mungo hypocotyl using a fluorescent probe, diS-C3-(5)

Kotaro Oka, Shigehiro Naitou, Motoko Yoshida, Hideo Ishikawa, Eiji Ohta, Makoto Sakata

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Membrane potentials of protoplasts isolated from Vigna mungo hypocotyl segments were measured using the fluorescent probe diS-C3-(5). The fluorescence intensity changed in response to the external K+ concentration. Membrane potential was estimated to be inside negative (-85±8 mV at 0.1 mM KCl) from the Nernst equation for K+. The membrane potential was not affected by DCCD (50 μM) or low temperature (5°C). Addition of 0.5 mM Ca2+ to the protoplast suspension markedly depolarized the membrane potential, and subsequent EDTA treatment repolarized it to the initial level. The Ca2+ effect on the membrane potential may be due to change in the permeability ratio of Cl- to K+.

本文言語English
ページ(範囲)843-849
ページ数7
ジャーナルPlant and Cell Physiology
28
5
DOI
出版ステータスPublished - 1987 7

ASJC Scopus subject areas

  • 生理学
  • 植物科学
  • 細胞生物学

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