Membrane-type 1 MMP (MMP-14) cleaves at three sites in the aggrecan interglobular domain

Amanda J. Fosang, Karena Last, Yutaka Fujii, Motoharu Seiki, Yasunori Okada

研究成果: Article査読

53 被引用数 (Scopus)

抄録

An aggrecan G1-G2 substrate was used to determine sites within the interglobular domain that were susceptible to cleavage by MT1-MMP. Degradation products were identified by Western blotting with neo-epitope antibodies specific for MMP-derived N- and C-terminal sequences. The results showed that MT1-MMP cleaved at the N341-F342 and D441-L442 bonds, as shown for other MMPs, and also at a site 13 amino acids C-terminal to the N341-F342 site. The G2 product of this additional cleavage was identified by sequence analysis and revealed an N-terminus commencing T355 VxxPDVELPLP. The data are consistent with MT1-MMP cleavage at three sites in the aggrecan interglobular domain; one at N342-F342, a second at D441-L442 and a third at Q354-T355.

本文言語English
ページ(範囲)186-190
ページ数5
ジャーナルFEBS Letters
430
3
DOI
出版ステータスPublished - 1998 7 3

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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