Mesenchymal to embryonic incomplete transition of human cells by chimeric OCT4/3 (POU5F1) with physiological co-activator EWS

Hatsune Makino, Masashi Toyoda, Kenji Matsumoto, Hirohisa Saito, Koichiro Nishino, Yoshihiro Fukawatase, Masakazu Machida, Hidenori Akutsu, Taro Uyama, Yoshitaka Miyagawa, Hajime Okita, Nobutaka Kiyokawa, Takashi Fujino, Yuichi Ishikawa, Takuro Nakamura, Akihiro Umezawa

研究成果: Article査読

47 被引用数 (Scopus)

抄録

POU5F1 (more commonly known as OCT4/3) is one of the stem cell markers, and affects direction of differentiation in embryonic stem cells. To investigate whether cells of mesenchymal origin acquire embryonic phenotypes, we generated human cells of mesodermal origin with overexpression of the chimeric OCT4/3 gene with physiological co-activator EWS (product of the EWSR1 gene), which is driven by the potent EWS promoter by translocation. The cells expressed embryonic stem cell genes such as NANOG, lost mesenchymal phenotypes, and exhibited embryonal stem cell-like alveolar structures when implanted into the subcutaneous tissue of immunodeficient mice. Hierarchical analysis by microchip analysis and cell surface analysis revealed that the cells are subcategorized into the group of human embryonic stem cells and embryonal carcinoma cells. These results imply that cells of mesenchymal origin can be traced back to cells of embryonic phenotype by the OCT4/3 gene in collaboration with the potent cis-regulatory element and the fused co-activator. The cells generated in this study with overexpression of chimeric OCT4/3 provide us with insight into cell plasticity involving OCT4/3 that is essential for embryonic cell maintenance, and the complexity required for changing cellular identity.

本文言語English
ページ(範囲)2727-2740
ページ数14
ジャーナルExperimental Cell Research
315
16
DOI
出版ステータスPublished - 2009 10 1
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

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