Metabolic profiling to identify potential serum biomarkers for gastric ulceration induced by nonsteroid anti-inflammatory drugs

Kenichiro Takeuchi, Maki Ohishi, Sana Ota, Kenichi Suzumura, Hitoshi Naraoka, Takeji Ohata, Jiro Seki, Youichi Miyamae, Masashi Honma, Tomoyoshi Soga

研究成果: Article査読

27 被引用数 (Scopus)

抄録

Nonsteroid anti-inflammatory drugs (NSAIDs) are among the most frequently prescribed drugs currently available. The most frequently reported serious side effects associated with NSAIDs are gastric mucosal ulceration and gastric hemorrhage. Presently, these side effects are only detectable by endoscopy, however, and no biomarkers have yet been identified. The ability to identify serum biomarkers would likely improve the safety of NSAID use. In this study we performed capillary electrophoresis-mass spectrometry (CE-MS)-based metabolomic profiling in stomach extract and serum from rats administered NSAIDs. Results showed drug-induced decreases in levels of citrate, cis-aconitate, succinate, 3-hydroxy butanoic acid, o-acetyl carnitine, proline, and hydroxyproline. We consider that these changes are due to NSAID-induced depression of mitochondrial function and activation of collagenase by lesions in the stomach. In addition, four of these changes in metabolite levels in the stomach were significantly correlated with changes in the serum. While further study is needed to clarify the mechanism of change in the level of these biomarkers, limitation of indications, and extrapolation to humans, these new serum biomarker candidates of gastric injury may be useful in the monitoring of NSAID-induced tissue damage.

本文言語English
ページ(範囲)1399-1407
ページ数9
ジャーナルJournal of Proteome Research
12
3
DOI
出版ステータスPublished - 2013 3

ASJC Scopus subject areas

  • 生化学
  • 化学 (全般)

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