Metabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantay

Patricio Martínez, Sebastián Gálvez, Norimasa Ohtsuka, Marko Budinich, María Paz Cortés, Cristián Serpell, Kenji Nakahigashi, Akiyoshi Hirayama, Masaru Tomita, Tomoyoshi Soga, Servet Martínez, Alejandro Maass, Pilar Parada

研究成果: Article

23 引用 (Scopus)

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In this study, we present the first metabolic profiles for two bioleaching bacteria using capillary electrophoresis coupled with mass spectrometry. The bacteria, Acidithiobacillus ferrooxidans strain Wenelen (DSM 16786) and Acidithiobacillus thiooxidans strain Licanantay (DSM 17318), were sampled at different growth phases and on different substrates: the former was grown with iron and sulfur, and the latter with sulfur and chalcopyrite. Metabolic profiles were scored from planktonic and sessile states. Spermidine was detected in intra- and extracellular samples for both strains, suggesting it has an important role in biofilm formation in the presence of solid substrate. The canonical pathway for spermidine synthesis seems absent as its upstream precursor, putrescine, was not present in samples. Glutathione, a catalytic activator of elemental sulfur, was identified as one of the most abundant metabolites in the intracellular space in A. thiooxidans strain Licanantay, confirming its participation in the sulfur oxidation pathway. Amino acid profiles varied according to the growth conditions and bioleaching species. Glutamic and aspartic acid were highly abundant in intra- and extracellular extracts. Both are constituents of the extracellular matrix, and have a probable role in cell detoxification. This novel metabolomic information validates previous knowledge from in silico metabolic reconstructions based on genomic sequences, and reveals important biomining functions such as biofilm formation, energy management and stress responses.

元の言語English
ページ(範囲)247-257
ページ数11
ジャーナルMetabolomics
9
発行部数1
DOI
出版物ステータスPublished - 2013

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry

フィンガープリント Metabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantay' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Martínez, P., Gálvez, S., Ohtsuka, N., Budinich, M., Cortés, M. P., Serpell, C., Nakahigashi, K., Hirayama, A., Tomita, M., Soga, T., Martínez, S., Maass, A., & Parada, P. (2013). Metabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantay. Metabolomics, 9(1), 247-257. https://doi.org/10.1007/s11306-012-0443-3