TY - JOUR
T1 - Methods and application of coherent X-ray diffraction imaging of noncrystalline particles
AU - Nakasako, Masayoshi
AU - Kobayashi, Amane
AU - Takayama, Yuki
AU - Asakura, Kenta
AU - Oide, Mao
AU - Okajima, Koji
AU - Oroguchi, Tomotaka
AU - Yamamoto, Masaki
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Microscopic imaging techniques have been developed to visualize events occurring in biological cells. Coherent X-ray diffraction imaging is one of the techniques applicable to structural analyses of cells and organelles, which have never been crystallized. In the experiment, a single noncrystalline particle is illuminated by an X-ray beam with almost complete spatial coherence. The structure of the particle projected along the direction of the beam is, in principle, retrieved from a finely recorded diffraction pattern alone by using iterative phase-retrieval algorithms. Here, we describe fundamental theory and experimental methods of coherent X-ray diffraction imaging and the recent application in structural studies of noncrystalline specimens by using X-rays available at Super Photon Ring of 8-Gev and SPring-8 Angstrom Compact Free Electron Laser in Japan.
AB - Microscopic imaging techniques have been developed to visualize events occurring in biological cells. Coherent X-ray diffraction imaging is one of the techniques applicable to structural analyses of cells and organelles, which have never been crystallized. In the experiment, a single noncrystalline particle is illuminated by an X-ray beam with almost complete spatial coherence. The structure of the particle projected along the direction of the beam is, in principle, retrieved from a finely recorded diffraction pattern alone by using iterative phase-retrieval algorithms. Here, we describe fundamental theory and experimental methods of coherent X-ray diffraction imaging and the recent application in structural studies of noncrystalline specimens by using X-rays available at Super Photon Ring of 8-Gev and SPring-8 Angstrom Compact Free Electron Laser in Japan.
KW - Coherent X-ray diffraction imaging
KW - Cryogenic experiment
KW - Noncrystalline particle
KW - Synchrotron radiation
KW - Three-dimensional structure
KW - X-ray free electron laser
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U2 - 10.1007/s12551-020-00690-9
DO - 10.1007/s12551-020-00690-9
M3 - Review article
AN - SCOPUS:85082778932
VL - 12
SP - 541
EP - 567
JO - Biophysical Reviews
JF - Biophysical Reviews
SN - 1867-2450
IS - 2
ER -