Micro-rotation flow chamber rapidly forming collagen gel-mediated hetero-spheroids

Hiroki Ota, Taiga Kodama, Masayuki Yamato, Teruo Okano, Norihisa Miki

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

Spheroids that are formed from aggregated cells enhance biological function compared to monolayer culture. In particular, hetero-spheroids composed of different types of cells, such as hepatocytes and endothelial cells, express tissue specific functions at a high level, which is advantageous for more precise drug screening and biological research. In this study, we propose rapid formation of three-dimensional hetero-spheroids consisting of hepatocytes and endothelial cells using micro-rotation flow. The hepatocytes are coated with collagen gel layers less than 200 nm thick to increase adhesion strength between hepatocytes and endothelial cells. Gel-coated hepatocytes and endothelial cells are collected in an array by micro-rotational flow and collagen-gel coating, thereby forming hetero-spheroids within 2 min. This array allowed the size of the three-dimensional spheroids to be hydrodynamically controlled by varying the cell density of the medium without altering the device geometry with standard deviations of less than 19%. The proposed microfulidic device, with its capacity of rapidly forming size-controlled hetero-cell aggregates, will offer an efficient experimental platform for heterospheroid study that will contribute to drug screening and regenerative medicine.

本文言語English
ホスト出版物のタイトル2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
出版社IEEE Computer Society
ページ94-98
ページ数5
ISBN(印刷版)9781457713613
DOI
出版ステータスPublished - 2011 1 1
イベント22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation - Nagoya, Japan
継続期間: 2011 11 62011 11 9

出版物シリーズ

名前2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"

Other

Other22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
CountryJapan
CityNagoya
Period11/11/611/11/9

ASJC Scopus subject areas

  • Artificial Intelligence
  • Mechanical Engineering

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