Microarray analysis of a reversible model and an irreversible model of anti-Thy-1 nephritis

M. Tsuji, Toshiaki Monkawa, J. Yoshino, M. Asai, S. Fukuda, H. Kawachi, F. Shimizu, M. Hayashi, T. Saruta

研究成果: Article

17 引用 (Scopus)

抄録

A single intravenous injection of anti-Thy-1 monoclonal antibody (mAb) 1-22-3 is known to cause reversible mesangial proliferative glomerulonephritis. However, mAb 1-22-3 injection followed by unilateral nephrectomy leads to progressive glomerulosclerosis and tubulointerstitial change with an irreversible course. To identify genes that play an important role in the irreversible progression of renal injury, we used microarray technology to identify differences in gene expression between these models. Rats were intravenously injected with mAb 1-22-3 1 week after unilateral nephrectomy (irreversible model) or a sham operation (reversible model), and rats were killed on days 4, 7, 14, 42, and 56 after the injection, complementary DNA probes prepared from kidney messenger RNAs were hybridized with oligonucleotide microarrays containing 4854 rat genes. The microarray identified 189 differentially expressed genes, having at least a two-fold difference in expression level between the two models, and they were classified into five clusters. One of the clusters consisted of genes whose expression was markedly upregulated in the irreversible model. This cluster included the genes encoding osteopontin, kidney injury molecule-1, and thymosin β10. Increased expression of thymosin β10 was localized mainly in macrophages in the fibrotic interstitium, and upregulation of thymosin β10 expression was also observed in a unilateral ureteral obstruction model. The microarray analysis yielded information on the molecular mechanisms responsible for the difference in disease progression between the reversible and irreversible model of anti-Thy-1 nephritis. Thymosin β10 may play an important role in the progression of kidney disease.

元の言語English
ページ(範囲)996-1004
ページ数9
ジャーナルKidney International
69
発行部数6
DOI
出版物ステータスPublished - 2006 3

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Thymosin
Nephritis
Microarray Analysis
Monoclonal Antibodies
Nephrectomy
Kidney
Genes
Gene Expression
Ureteral Obstruction
Injections
Osteopontin
DNA Probes
Wounds and Injuries
Kidney Diseases
Multigene Family
Glomerulonephritis
Oligonucleotide Array Sequence Analysis
Intravenous Injections
Disease Progression
Up-Regulation

ASJC Scopus subject areas

  • Nephrology

これを引用

Microarray analysis of a reversible model and an irreversible model of anti-Thy-1 nephritis. / Tsuji, M.; Monkawa, Toshiaki; Yoshino, J.; Asai, M.; Fukuda, S.; Kawachi, H.; Shimizu, F.; Hayashi, M.; Saruta, T.

:: Kidney International, 巻 69, 番号 6, 03.2006, p. 996-1004.

研究成果: Article

Tsuji, M, Monkawa, T, Yoshino, J, Asai, M, Fukuda, S, Kawachi, H, Shimizu, F, Hayashi, M & Saruta, T 2006, 'Microarray analysis of a reversible model and an irreversible model of anti-Thy-1 nephritis', Kidney International, 巻. 69, 番号 6, pp. 996-1004. https://doi.org/10.1038/sj.ki.5000191
Tsuji, M. ; Monkawa, Toshiaki ; Yoshino, J. ; Asai, M. ; Fukuda, S. ; Kawachi, H. ; Shimizu, F. ; Hayashi, M. ; Saruta, T. / Microarray analysis of a reversible model and an irreversible model of anti-Thy-1 nephritis. :: Kidney International. 2006 ; 巻 69, 番号 6. pp. 996-1004.
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