@article{66daba91e3ff4cf48a5366eedfc3b43d,
title = "Microbiota-derived lantibiotic restores resistance against vancomycin-resistant Enterococcus",
abstract = "Intestinal commensal bacteria can inhibit dense colonization of the gut by vancomycin-resistant Enterococcus faecium (VRE), a leading cause of hospital-acquired infections1,2. A four-strained consortium of commensal bacteria that contains Blautia producta BPSCSK can reverse antibiotic-induced susceptibility to VRE infection3. Here we show that BPSCSK reduces growth of VRE by secreting a lantibiotic that is similar to the nisin-A produced by Lactococcus lactis. Although the growth of VRE is inhibited by BPSCSK and L. lactis in vitro, only BPSCSK colonizes the colon and reduces VRE density in vivo. In comparison to nisin-A, the BPSCSK lantibiotic has reduced activity against intestinal commensal bacteria. In patients at high risk of VRE infection, high abundance of the lantibiotic gene is associated with reduced density of E. faecium. In germ-free mice transplanted with patient-derived faeces, resistance to VRE colonization correlates with abundance of the lantibiotic gene. Lantibiotic-producing commensal strains of the gastrointestinal tract reduce colonization by VRE and represent potential probiotic agents to re-establish resistance to VRE.",
author = "Kim, {Sohn G.} and Simone Becattini and Moody, {Thomas U.} and Shliaha, {Pavel V.} and Littmann, {Eric R.} and Ruth Seok and Mergim Gjonbalaj and Vincent Eaton and Emily Fontana and Luigi Amoretti and Roberta Wright and Silvia Caballero and Wang, {Zhong Min X.} and Jung, {Hea Jin} and Morjaria, {Sejal M.} and Leiner, {Ingrid M.} and Weige Qin and Ramos, {Ruben J.J.F.} and Cross, {Justin R.} and Seiko Narushima and Kenya Honda and Peled, {Jonathan U.} and Hendrickson, {Ronald C.} and Ying Taur and {van den Brink}, {Marcel R.M.} and Pamer, {Eric G.}",
note = "Funding Information: Acknowledgements This work was supported by grants RO1 AI42135, RO1 AI95706, UO1 AI124275, and P30 CA008748 from the US National Institutes of Health (NIH) and the Tow Foundation and Lucille Castori Center for Microbes, Inflammation and Cancer to E.G.P. S.G.K. is supported by a Medical Scientist Training Program grant from the National Institute of General Medical Sciences, NIH (award T32GM07739 to the Weill Cornell/Rockefeller/ Sloan Kettering Tri-Institutional MD-PhD Program). S.B. was supported by an Early Postdoc Mobility Fellowship from the Swiss National Science Foundation and an Irvington Fellowship from the Cancer Research Institute. We thank members of the Pamer laboratory for discussions and comments on the manuscript. Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2019",
month = aug,
day = "29",
doi = "10.1038/s41586-019-1501-z",
language = "English",
volume = "572",
pages = "665--669",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "7771",
}