TY - JOUR
T1 - Microcoaxial electrode for local nitric oxide measurement in biological systems
AU - Kitamura, Yoshiichiro
AU - Uzawa, Tomoyuki
AU - Oka, Kotaro
AU - Komai, Yutaka
AU - Ogawa, Hiroto
AU - Kobayashi, Hirosuke
AU - Takizawa, Naosada
AU - Tanishita, Kazuo
PY - 1998
Y1 - 1998
N2 - A new nitric oxide (NO) selective electrode was developed. The tip diameter of the NO electrode is less than 10 μm. A working and a reference electrodes are located coaxially at the tip of the NO electrode. A stable NO donor SNAP (S-nitroso-N-acetyl-DL-penicillamine) and a putative NO scavenger hemoglobin (Hb) was used to examine the response of the electrode. Considering the instantaneous concentration of NO secretion previously reported in biological systems, 0.1, 1 μM NO solution was used to calibrate the electrode. From the result of the test of the electrode with SNAP and Hb, we confirmed this microcoaxial NO electrode can detect NO released from SNAP directly and continuously. From the result of calibration of the electrode, good linearity was examined in sub-molar concentration range. It is expected to detect local NO concentration in biological systems with a high space resolution.
AB - A new nitric oxide (NO) selective electrode was developed. The tip diameter of the NO electrode is less than 10 μm. A working and a reference electrodes are located coaxially at the tip of the NO electrode. A stable NO donor SNAP (S-nitroso-N-acetyl-DL-penicillamine) and a putative NO scavenger hemoglobin (Hb) was used to examine the response of the electrode. Considering the instantaneous concentration of NO secretion previously reported in biological systems, 0.1, 1 μM NO solution was used to calibrate the electrode. From the result of the test of the electrode with SNAP and Hb, we confirmed this microcoaxial NO electrode can detect NO released from SNAP directly and continuously. From the result of calibration of the electrode, good linearity was examined in sub-molar concentration range. It is expected to detect local NO concentration in biological systems with a high space resolution.
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M3 - Article
AN - SCOPUS:0032304216
SN - 1071-6947
VL - 39
SP - 387
EP - 388
JO - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
JF - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
ER -