Microfluidic experimental platform for producing size-controlled three-dimensional spheroids

Hiroki Ota, Norihisa Miki

研究成果: Article査読

33 被引用数 (Scopus)

抄録

We propose a microfluidic experimental platform for producing size-controlled spheroids. Cells were aggregated into chambers arranged in an array by microrotational flow within 120 s to form spheroids. The cell density of the initial medium and hydrodynamic flow in the developed array could be adjusted while keeping the device geometry the same to control spheroid size with a standard deviation of less than 19% of the mean. Using this device, spheroids of HepG2 cells of various size categories could be maintained for three days in the chamber with medium exchange and could be continuously evaluated for topology and hepatic functions. Furthermore, CYP1A1 activities were found to increase with time to a constant level at three days. These results demonstrate that this device is readily applicable to producing and maintaining spheroids for in vitro drug screening and biological research.

本文言語English
ページ(範囲)266-273
ページ数8
ジャーナルSensors and Actuators, A: Physical
169
2
DOI
出版ステータスPublished - 2011 10

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学

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