Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.
|出版ステータス||Published - 2008 1月 1|
|イベント||12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States|
継続期間: 2008 10月 12 → 2008 10月 16
|Other||12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008|
|City||San Diego, CA|
|Period||08/10/12 → 08/10/16|
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