Microglial phospholipase D4 deficiency influences myelination during brain development

Terumasa Chiba, Yoshinori Otani, Yoshihide Yamaguchi, Tomoko Ishibashi, Akiko Hayashi, Kenji F. Tanaka, Maya Yamazaki, Kenji Sakimura, Hiroko Baba

研究成果: Article

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Phospholipase D4 (PLD4) is expressed in activated microglia that transiently appear in white matter during postnatal brain development. Previous knockdown experiments using cultured microglia showed PLD4 involvement in phagocytosis and proliferation. To elucidate the role of PLD4 in vivo, PLD4-deficient mice were generated and the cerebella were examined at postnatal day 5 (P5) and P7, when PLD4 expression is highest in microglia. Wild type microglia showed strong immunoreactivity for microglial marker CD68 at P5, whereas CD68 signals were weak in PLD4-deficient microglia, suggesting that loss of PLD4 affects microglial activation. At P5 and P7, immunostaining for anti-myelin basic protein (MBP) antibody indicated a mild but significant delay in myelination in PLD4-deficient cerebellum. Similar change was also observed in the corpus callosum at P7. However, this difference was not apparent at P10, suggesting that microglial PLD4-deficiency primarily influences the early myelination stage. Thus, microglia may have a transient role in myelination via a PLD4-related mechanism during development.

元の言語English
ページ(範囲)237-254
ページ数18
ジャーナルProceedings of the Japan Academy Series B: Physical and Biological Sciences
92
発行部数7
DOI
出版物ステータスPublished - 2016

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Physics and Astronomy(all)

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