Micro/nano-integrated fluidic device for living single-cell protein analysis

Tatsuro Nakao, Yutaka Kazoe, Kyojiro Morikawa, Ayumi Yoshizaki, Kazuma Mawatari, Takehiko Kitamori

研究成果: Conference contribution

抄録

Based on our concept of living single-cell protein analysis where single cell is processed in microchannel and secreted proteins are analyzed in nanochannel, we developed a micro/nano-integrated fluidic device where micro/nanochannels are hierarchically structured. All the 11 unit operations of single-cell analysis including single-cell isolation, femtoliter sampling, antigen-antibody reaction are integrated in femtoliter-to-picoliter space, and the working principle was verified by detecting a signal from 11 fL volume of 75 nM standard C-reactive protein solution (~500 molecules). This device can contribute to medicine and biology by providing a novel tool for elucidation of single-cell-level mechanism of cancer or immune diseases.

本文言語English
ホスト出版物のタイトル22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
出版社Chemical and Biological Microsystems Society
ページ132-134
ページ数3
ISBN(電子版)9781510897571
出版ステータスPublished - 2018
外部発表はい
イベント22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
継続期間: 2018 11 112018 11 15

出版物シリーズ

名前22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
1

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period18/11/1118/11/15

ASJC Scopus subject areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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