Microvascular sprouting, extension, and creation of new capillary connections with adaptation of the neighboring astrocytes in adult mouse cortex under chronic hypoxia

Kazuto Masamoto, Hiroyuki Takuwa, Chie Seki, Junko Taniguchi, Yoshiaki Itoh, Yutaka Tomita, Haruki Toriumi, Miyuki Unekawa, Hiroshi Kawaguchi, Hiroshi Ito, Norihiro Suzuki, Iwao Kanno

研究成果: Article査読

23 被引用数 (Scopus)

抄録

The present study aimed to determine the spatiotemporal dynamics of microvascular and astrocytic adaptation during hypoxia-induced cerebral angiogenesis. Adult C57BL/6J and Tie2-green fluorescent protein (GFP) mice with vascular endothelial cells expressing GFP were exposed to normobaric hypoxia for 3 weeks, whereas the three-dimensional microvessels and astrocytes were imaged repeatedly using two-photon microscopy. After 7 to14 days of hypoxia, a vessel sprout appeared from the capillaries with a bump-like head shape (mean diameter 14 μm), and stagnant blood cells were seen inside the sprout. However, no detectable changes in the astrocyte morphology were observed for this early phase of the hypoxia adaptation. More than 50% of the sprouts emerged from capillaries 60 μm away from the center penetrating arteries, which indicates that the capillary distant from the penetrating arteries is a favored site for sprouting. After 14 to 21 days of hypoxia, the sprouting vessels created a new connection with an existing capillary. In this phase, the shape of the new vessel and its blood flow were normalized, and the outside of the vessels were wrapped with numerous processes from the neighboring astrocytes. The findings indicate that hypoxia-induced cerebral angiogenesis provokes the adaptation of neighboring astrocytes, which may stabilize the blood-brain barrier in immature vessels.

本文言語English
ページ(範囲)325-331
ページ数7
ジャーナルJournal of Cerebral Blood Flow and Metabolism
34
2
DOI
出版ステータスPublished - 2014 2
外部発表はい

ASJC Scopus subject areas

  • 神経学
  • 臨床神経学
  • 循環器および心血管医学

フィンガープリント

「Microvascular sprouting, extension, and creation of new capillary connections with adaptation of the neighboring astrocytes in adult mouse cortex under chronic hypoxia」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル