Mini-Gut Organoids: Reconstitution of the Stem Cell Niche

Shoichi Date, Toshiro Sato

研究成果: Article査読

134 被引用数 (Scopus)


In the adult mammalian body, self-renewal of tissue stem cells is regulated by extracellular niche environments in response to the demands of tissue organization. Intestinal stem cells expressing Lgr5 constantly self-renew in their specific niche at the crypt bottom to maintain rapid turnover of the epithelium. Niche-regulated stem cell self-renewal is perturbed in several mouse genetic models and during human tumorigenesis, suggesting roles for EGF, Wnt, BMPTGF-β, and Notch signaling. In vitro niche reconstitution capitalizing on this knowledge has enabled the growth of single intestinal stem cells into mini-gut epithelial organoids comprising Lgr5+ stem cells and all types of differentiated lineages. The mini-gut organoid culture platform is applicable to various types of digestive tissue epithelium from multiple species. The mechanism of self-renewal in organoids provides novel insights for organogenesis, regenerative medicine, and tumorigenesis of the digestive system.

ジャーナルAnnual Review of Cell and Developmental Biology
出版ステータスPublished - 2015 11月 13

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学


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