miR-363 induces transdifferentiation of human kidney tubular cells to mesenchymal phenotype

Ryuji Morizane, Shizuka Fujii, Toshiaki Monkawa, Ken Hiratsuka, Shintaro Yamaguchi, Koichiro Honma, Hiroshi Itoh

研究成果: Article

5 引用 (Scopus)

抄録

Background: microRNAs (miRNAs) are non-coding small RNAs that regulate embryonic development, cell differentiation and pathological processes via interaction with mRNA. Epithelial–mesenchymal transition (EMT) is pathological process that involves in a variety of diseases such as cancer or fibrosis. Methods: In this study, we identified miR-363 as a potent inducer of EMT by microarray analysis in human kidney tubular cells, and analyzed the function and mechanisms of miR-363. Results: Overexpression of miR-363 induced mesenchymal phenotypes with loss of epithelial phenotypes in human kidney tubular cells. In addition, in vitro scratch assay demonstrated that miR-363 promotes cell migration of primary culture of human kidney tubular cells. We identified TWIST/canonical WNT pathway as the downstream effecter of miR-363, and inhibition of canonical WNT by small molecule, IWR-1, attenuated EMT induced by miR-363. Conclusion: miR-363 induces transdifferentiation of human kidney tubular cells via upregulation of TWIST/canonical WNT pathway.

元の言語English
ジャーナルClinical and Experimental Nephrology
DOI
出版物ステータスAccepted/In press - 2015 9 15

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Phenotype
Kidney
Pathologic Processes
Small Untranslated RNA
Microarray Analysis
MicroRNAs
Embryonic Development
Cell Movement
Cell Differentiation
Fibrosis
Up-Regulation
Messenger RNA
Neoplasms
In Vitro Techniques

ASJC Scopus subject areas

  • Nephrology
  • Physiology
  • Physiology (medical)

これを引用

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title = "miR-363 induces transdifferentiation of human kidney tubular cells to mesenchymal phenotype",
abstract = "Background: microRNAs (miRNAs) are non-coding small RNAs that regulate embryonic development, cell differentiation and pathological processes via interaction with mRNA. Epithelial–mesenchymal transition (EMT) is pathological process that involves in a variety of diseases such as cancer or fibrosis. Methods: In this study, we identified miR-363 as a potent inducer of EMT by microarray analysis in human kidney tubular cells, and analyzed the function and mechanisms of miR-363. Results: Overexpression of miR-363 induced mesenchymal phenotypes with loss of epithelial phenotypes in human kidney tubular cells. In addition, in vitro scratch assay demonstrated that miR-363 promotes cell migration of primary culture of human kidney tubular cells. We identified TWIST/canonical WNT pathway as the downstream effecter of miR-363, and inhibition of canonical WNT by small molecule, IWR-1, attenuated EMT induced by miR-363. Conclusion: miR-363 induces transdifferentiation of human kidney tubular cells via upregulation of TWIST/canonical WNT pathway.",
keywords = "Cancer, EMT, Fibrosis, Kidney, miR-363, miRNA",
author = "Ryuji Morizane and Shizuka Fujii and Toshiaki Monkawa and Ken Hiratsuka and Shintaro Yamaguchi and Koichiro Honma and Hiroshi Itoh",
year = "2015",
month = "9",
day = "15",
doi = "10.1007/s10157-015-1167-2",
language = "English",
journal = "Clinical and Experimental Nephrology",
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TY - JOUR

T1 - miR-363 induces transdifferentiation of human kidney tubular cells to mesenchymal phenotype

AU - Morizane, Ryuji

AU - Fujii, Shizuka

AU - Monkawa, Toshiaki

AU - Hiratsuka, Ken

AU - Yamaguchi, Shintaro

AU - Honma, Koichiro

AU - Itoh, Hiroshi

PY - 2015/9/15

Y1 - 2015/9/15

N2 - Background: microRNAs (miRNAs) are non-coding small RNAs that regulate embryonic development, cell differentiation and pathological processes via interaction with mRNA. Epithelial–mesenchymal transition (EMT) is pathological process that involves in a variety of diseases such as cancer or fibrosis. Methods: In this study, we identified miR-363 as a potent inducer of EMT by microarray analysis in human kidney tubular cells, and analyzed the function and mechanisms of miR-363. Results: Overexpression of miR-363 induced mesenchymal phenotypes with loss of epithelial phenotypes in human kidney tubular cells. In addition, in vitro scratch assay demonstrated that miR-363 promotes cell migration of primary culture of human kidney tubular cells. We identified TWIST/canonical WNT pathway as the downstream effecter of miR-363, and inhibition of canonical WNT by small molecule, IWR-1, attenuated EMT induced by miR-363. Conclusion: miR-363 induces transdifferentiation of human kidney tubular cells via upregulation of TWIST/canonical WNT pathway.

AB - Background: microRNAs (miRNAs) are non-coding small RNAs that regulate embryonic development, cell differentiation and pathological processes via interaction with mRNA. Epithelial–mesenchymal transition (EMT) is pathological process that involves in a variety of diseases such as cancer or fibrosis. Methods: In this study, we identified miR-363 as a potent inducer of EMT by microarray analysis in human kidney tubular cells, and analyzed the function and mechanisms of miR-363. Results: Overexpression of miR-363 induced mesenchymal phenotypes with loss of epithelial phenotypes in human kidney tubular cells. In addition, in vitro scratch assay demonstrated that miR-363 promotes cell migration of primary culture of human kidney tubular cells. We identified TWIST/canonical WNT pathway as the downstream effecter of miR-363, and inhibition of canonical WNT by small molecule, IWR-1, attenuated EMT induced by miR-363. Conclusion: miR-363 induces transdifferentiation of human kidney tubular cells via upregulation of TWIST/canonical WNT pathway.

KW - Cancer

KW - EMT

KW - Fibrosis

KW - Kidney

KW - miR-363

KW - miRNA

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U2 - 10.1007/s10157-015-1167-2

DO - 10.1007/s10157-015-1167-2

M3 - Article

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AN - SCOPUS:84941702722

JO - Clinical and Experimental Nephrology

JF - Clinical and Experimental Nephrology

SN - 1342-1751

ER -