Mitosis in beating cardiac muscle cells from newt embryos in vitro

Hiroyuki Kaneko, S. I. Abe, S. Ito

研究成果: Article

12 引用 (Scopus)


Cardiac muscle cells from newt embryos were cultured at relatively low cell density. Within 10 days in culture, 2 cell types (spindle and flat type) were distinguished both among beating and non-beating cells. Mitosis in single beating cells was frequently observed both in spindle and flat cells. Some cells maintained almost constant contractile activities throughout the mitotic stages, while the others transiently stopped beating during mitosis, which accords well to the case in chick embryos. Ultra-thin section shows the presence of myofibril's structure in a dividing cell, as shown in newborn rats, chick embryos and adult newts. As a consequence of mitosis, 3 types (spindle, flat and mixed type) of beating colonies developed after 3-4 weeks in culture. Cell proliferation was accompanied by pulsation and could be directly pursued till the 4th division, suggesting that differentiated myocardiac cells with myofibrils proliferate by their mitoses in vivo, maintaining rhythmic contraction.

ジャーナルDevelopment Growth and Differentiation
出版物ステータスPublished - 1981


ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Anatomy