Modeling Human Digestive Diseases With CRISPR-Cas9–Modified Organoids

Masayuki Fujii, Hans Clevers, Toshiro Sato

研究成果: Review article

8 引用 (Scopus)

抄録

Insights into the stem cell niche have allowed researchers to cultivate adult tissue stem cells as organoids that display structural and phenotypic features of healthy and diseased epithelial tissues. Organoids derived from patients’ tissues are used as models of disease and to test drugs. CRISPR-Cas9 technology can be used to genetically engineer organoids for studies of monogenic diseases and cancer. We review the derivation of organoids from human gastrointestinal tissues and how CRISPR-Cas9 technology can be used to study these organoids. We discuss burgeoning technologies that are broadening our understanding of diseases of the digestive system.

元の言語English
ページ(範囲)562-576
ページ数15
ジャーナルGastroenterology
156
発行部数3
DOI
出版物ステータスPublished - 2019 2 1

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Clustered Regularly Interspaced Short Palindromic Repeats
Organoids
Technology
Digestive System Diseases
Stem Cell Niche
Adult Stem Cells
Epithelium
Research Personnel
Pharmaceutical Preparations
Neoplasms

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

これを引用

Modeling Human Digestive Diseases With CRISPR-Cas9–Modified Organoids. / Fujii, Masayuki; Clevers, Hans; Sato, Toshiro.

:: Gastroenterology, 巻 156, 番号 3, 01.02.2019, p. 562-576.

研究成果: Review article

Fujii, Masayuki ; Clevers, Hans ; Sato, Toshiro. / Modeling Human Digestive Diseases With CRISPR-Cas9–Modified Organoids. :: Gastroenterology. 2019 ; 巻 156, 番号 3. pp. 562-576.
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