Modification of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy

Tae Hwa Chun, Hiroshi Itoh, Lalitha Subramanian, Jorge A. Iñiguez-Lluhí, Kazuwa Nakao

研究成果: Article査読

51 被引用数 (Scopus)

抄録

GATA sequences are required for the optimal expression of endothelial cell-specific genes, including endothelin-1 (ET-1). We have identified PIASy in a search for new GATA-2 interacting proteins that can regulate GATA-2-mediated endothelial gene expression. Notably, among the cell populations comprising vascular walls, PIASy mRNA is selectively expressed in endothelial cells, and its expression can be regulated by angiogenic growth factors. We show that GATA-2 is covalently modified by small ubiquitin-like modifier (SUMO)-1 and -2 and that PIASy, through its E3 SUMO ligase activity, preferentially enhances the conjugation of SUMO-2 to GATA-2. Through a functional analysis, we demonstrate that PIASy potently suppresses the activity of the GATA-2-dependent human ET-1 promoter in endothelial cells. The suppressive effect of PIASy requires the GATA-binding site in the ET-1 promoter and depends on its interaction with GATA-2, which requires both N-terminal (amino acids 1-183) and C-terminal (amino acids 414-510) sequences in PIASy. We conclude that PIASy enhances the conjugation of SUMO-2 to GATA-2 and that the interaction of PIASy with GATA-2 can modulate GATA-mediated ET-1 transcription activity in endothelial cells through a RING-like domain-independent mechanism.

本文言語English
ページ(範囲)1201-1208
ページ数8
ジャーナルCirculation research
92
11
DOI
出版ステータスPublished - 2003 6 13
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 循環器および心血管医学

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