Modulation of collagen synthesis by tumor necrosis factor alpha in cultured vascular smooth muscle cells

Syouichi Hiraga, Toshiyuki Kaji, Yoshimichi Ueda, Fumiko Zisaki, Kazushi Iwata, Fumitomo Koizumi, Yasunori Okada, Shogo Katsuda, Isao Nakanishi

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Collagen synthesis in vascular smooth muscle cells (SMCs) after exposure to tumor necrosis factor alpha (TNF-α) was investigated using a culture system. The synthesis of collagenase-digestible proteins (CDP) and noncollagenous proteins (NCP) was evaluated by the [3H]proline incorporation. It was shown that TNF-α markedly suppresses the incorporation of [3H]proline into both CDP and NCP in confluent cultures of SMCs but not in sparse cultures of the cells. Such a marked suppression by TNF-α was not observed in confluent bovine aortic endothelial cells and human fibroblastic IMR-90 cells. In confluent SMCs, the synthesis of CDP was more strongly inhibited by TNF-α than that of NCP. When the CDP synthesis was stimulated by transforming growth factor beta, TNF-α suppressed the stimulation in both confluent and sparse SMCs. Human SMCs synthesized types I, III, IV and V collagens; TNF-α markedly decreased the relative proportion of types IV and V. It was therefore suggested that TNF-α modulates the collagen synthesis by SMCs depending on their cell density and modifies the formation of atherosclerotic lesions.

本文言語English
ページ(範囲)235-244
ページ数10
ジャーナルLife Sciences
66
3
DOI
出版ステータスPublished - 1999 12 10
外部発表はい

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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