Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus

Hiroshi Kagoshima, Satoshi Imura, Atsushi C Suzuki

研究成果: Article

9 引用 (Scopus)

抄録

We isolated a species of tardigrade from moss samples collected from Langhovde and Skarvsnes, near Syowa station, East Antarctic, from which we cultured a parthenogenetic strain in Petri dishes with co-occurring cyanobacteria or green algae. This culture was maintained at both 4 and 10°C, though the latter proved more suitable for growth. Eggs were laid free, rather than in exuviae. We isolated the 18S rRNA sequences from this tardigrade, identical to that ofacutuncus antarcticusfrom King George island, South Shetland islands. Morphological analyses via both light and scanning electron microscopy also show general agreement with characteristics of a. antarcticus: dorsal and ventral apophyses for the insertion of stylet muscles and dorsal longitudinal thickening on the anterior part of buccal tube; presence of pharyngeal apophyses, two macroplacoids and absence of a microplacoid; the surface structure of egg; and claw shape. Peribuccal lamellae were absent, but six oval swellings surrounded the mouth opening. An additional study of moss pillars from lake Hotoke-ike, Skarvsnes, proved the existence of the same tardigrade taxon living at the bottom of the lake.

元の言語English
ページ(範囲)15-23
ページ数9
ジャーナルJournal of Limnology
72
発行部数SUPPL 1
DOI
出版物ステータスPublished - 2013

Fingerprint

Tardigrada
moss
egg
mosses and liverworts
lake
pillar
green alga
swelling
cyanobacterium
lakes
muscle
stylets
scanning electron microscopy
claws
integument
Antarctica
Chlorophyta
Cyanobacteria
mouth
ribosomal RNA

ASJC Scopus subject areas

  • Water Science and Technology
  • Aquatic Science
  • Ecology

これを引用

Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus. / Kagoshima, Hiroshi; Imura, Satoshi; Suzuki, Atsushi C.

:: Journal of Limnology, 巻 72, 番号 SUPPL 1, 2013, p. 15-23.

研究成果: Article

Kagoshima, Hiroshi ; Imura, Satoshi ; Suzuki, Atsushi C. / Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus. :: Journal of Limnology. 2013 ; 巻 72, 番号 SUPPL 1. pp. 15-23.
@article{116c8ed2bcb74942af11010c5939452f,
title = "Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus",
abstract = "We isolated a species of tardigrade from moss samples collected from Langhovde and Skarvsnes, near Syowa station, East Antarctic, from which we cultured a parthenogenetic strain in Petri dishes with co-occurring cyanobacteria or green algae. This culture was maintained at both 4 and 10°C, though the latter proved more suitable for growth. Eggs were laid free, rather than in exuviae. We isolated the 18S rRNA sequences from this tardigrade, identical to that ofacutuncus antarcticusfrom King George island, South Shetland islands. Morphological analyses via both light and scanning electron microscopy also show general agreement with characteristics of a. antarcticus: dorsal and ventral apophyses for the insertion of stylet muscles and dorsal longitudinal thickening on the anterior part of buccal tube; presence of pharyngeal apophyses, two macroplacoids and absence of a microplacoid; the surface structure of egg; and claw shape. Peribuccal lamellae were absent, but six oval swellings surrounded the mouth opening. An additional study of moss pillars from lake Hotoke-ike, Skarvsnes, proved the existence of the same tardigrade taxon living at the bottom of the lake.",
keywords = "18S rRNA, Antarctica, Cultivation, Exuviae, JARE (Japanese Antarctic Research Expedition), Tardigrada",
author = "Hiroshi Kagoshima and Satoshi Imura and Suzuki, {Atsushi C}",
year = "2013",
doi = "10.4081/jlimnol.2013.s1.e3",
language = "English",
volume = "72",
pages = "15--23",
journal = "Journal of Limnology",
issn = "1129-5767",
publisher = "PagePress",
number = "SUPPL 1",

}

TY - JOUR

T1 - Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus

AU - Kagoshima, Hiroshi

AU - Imura, Satoshi

AU - Suzuki, Atsushi C

PY - 2013

Y1 - 2013

N2 - We isolated a species of tardigrade from moss samples collected from Langhovde and Skarvsnes, near Syowa station, East Antarctic, from which we cultured a parthenogenetic strain in Petri dishes with co-occurring cyanobacteria or green algae. This culture was maintained at both 4 and 10°C, though the latter proved more suitable for growth. Eggs were laid free, rather than in exuviae. We isolated the 18S rRNA sequences from this tardigrade, identical to that ofacutuncus antarcticusfrom King George island, South Shetland islands. Morphological analyses via both light and scanning electron microscopy also show general agreement with characteristics of a. antarcticus: dorsal and ventral apophyses for the insertion of stylet muscles and dorsal longitudinal thickening on the anterior part of buccal tube; presence of pharyngeal apophyses, two macroplacoids and absence of a microplacoid; the surface structure of egg; and claw shape. Peribuccal lamellae were absent, but six oval swellings surrounded the mouth opening. An additional study of moss pillars from lake Hotoke-ike, Skarvsnes, proved the existence of the same tardigrade taxon living at the bottom of the lake.

AB - We isolated a species of tardigrade from moss samples collected from Langhovde and Skarvsnes, near Syowa station, East Antarctic, from which we cultured a parthenogenetic strain in Petri dishes with co-occurring cyanobacteria or green algae. This culture was maintained at both 4 and 10°C, though the latter proved more suitable for growth. Eggs were laid free, rather than in exuviae. We isolated the 18S rRNA sequences from this tardigrade, identical to that ofacutuncus antarcticusfrom King George island, South Shetland islands. Morphological analyses via both light and scanning electron microscopy also show general agreement with characteristics of a. antarcticus: dorsal and ventral apophyses for the insertion of stylet muscles and dorsal longitudinal thickening on the anterior part of buccal tube; presence of pharyngeal apophyses, two macroplacoids and absence of a microplacoid; the surface structure of egg; and claw shape. Peribuccal lamellae were absent, but six oval swellings surrounded the mouth opening. An additional study of moss pillars from lake Hotoke-ike, Skarvsnes, proved the existence of the same tardigrade taxon living at the bottom of the lake.

KW - 18S rRNA

KW - Antarctica

KW - Cultivation

KW - Exuviae

KW - JARE (Japanese Antarctic Research Expedition)

KW - Tardigrada

UR - http://www.scopus.com/inward/record.url?scp=84880794197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880794197&partnerID=8YFLogxK

U2 - 10.4081/jlimnol.2013.s1.e3

DO - 10.4081/jlimnol.2013.s1.e3

M3 - Article

AN - SCOPUS:84880794197

VL - 72

SP - 15

EP - 23

JO - Journal of Limnology

JF - Journal of Limnology

SN - 1129-5767

IS - SUPPL 1

ER -