Molecular Cloning and Characterization of the HmrM Multidrug Efflux Pump from Haemophilus influenzae Rd

Xing Jue Xu, Xian Zhong Su, Yuji Morita, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

研究成果: Article査読

37 被引用数 (Scopus)

抄録

We cloned a gene responsible for norfloxacin resistance from the chromosomal DNA of Haemophilus influenzae Rd, and designated the gene as hmrM. HmrM showed sequence similarity with NorM of Vibrio parahaemolyticus and YdhE of Escherichia coli and others that belong to the MATE family multidrug efflux pumps. The recombinant plasmid carrying the hmrM gene conferred elevated resistance not only to norfloxacin but also to acriflavine, 4′ ,6-diamidino-2-phenylindole, doxorubicin, ethidium bromide, tetraphenylphosphonium chloride, Hoechst 33342, daunomycin, berberine, and sodium deoxycholate in Escherichia coli KAM32, a drug-hypersensitive strain. We observed an Na+-dependent efflux of ethidium and an ethidium-induced efflux of Na+ in E. coli KAM32 cells harboring the plasmid carrying the hmrM gene. These results indicate that HmrM is an Na+/drug antiporter-type multidrug efflux pump. A difference in substrate preference was observed between HmrM, NorM, and YdhE.

本文言語English
ページ(範囲)937-943
ページ数7
ジャーナルMicrobiology and Immunology
47
12
DOI
出版ステータスPublished - 2003

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • ウイルス学

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