Molecular cloning and characterization of the inositol 1,4,5-trisphosphate receptor in Drosophila melanogaster

S. Yoshikawa, T. Tanimura, A. Miyawaki, M. Nakamura, M. Yuzaki, T. Furuichi, K. Mikoshiba

研究成果: Article査読

134 被引用数 (Scopus)

抄録

We isolated a cDNA encoding an inositol 1,4,5-trisphosphate receptor (InsP3R) of Drosophila melanogaster. The predicted Drosophila InsP3R (2,833 amino acids) has extensive sequence similarity to the mouse InsP3R. The polypeptide encoded by the cDNA was functionally expressed and showed characteristic InsP3-binding activity. The Drosophila InsP3R gene is located at the region 83A5-9 on the third chromosome and expresses throughout development but predominantly in the adult. Localization of the InsP3R mRNA in adult tissues suggests strong expression in the retina and antenna, indicating the involvement of the InsP3R in visual and olfactory transduction. In addition, the InsP3R mRNA is abundant in the legs and thorax, which are enriched with a muscular system. Such localization is apparently consistent with the quantitatively predominant sites for [3H]InsP3 binding in Drosophila and the fleshfly (Boettcherisca peregrina). The present study points to the likely functional importance of the InsP3/Ca2+ signaling system in Drosophila.

本文言語English
ページ(範囲)16613-16619
ページ数7
ジャーナルJournal of Biological Chemistry
267
23
出版ステータスPublished - 1992
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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