Molecular cloning, expression and characterization of a phenol sulfotransferase cDNA from mouse intestine

Hiro'omi Tamura, Atsushi Miyawaki, Hiroyuki Yoneshima, Katsuhiko Mikoshiba, Michio Matsui

研究成果: Article

6 引用 (Scopus)

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A sulfotransferase (ST) cDNA was isolated from a mouse intestinal cDNA library using a probe which was generated by reverse transcription (RT)-PCR based on the conserved amino acid sequences of the ST molecules. The isolated cDNA (1.1 kb) contained an 858 bp open reading frame encoding a 286 amino acid polypeptide with molecular weight of 33439. The deduced amino acid sequence shares 55.1% and 40.2% identity with mouse liver aryl/phenol (mSTp1) and alcohol (mSTa1 or mSTa2) STs, respectively, and it is highly similar to those of rat and human liver phenol ST (P-ST) isozymes, ST1B1 (87.8%) and ST1B2 (71.0%), respectively. RT-PCR analyses showed abundant expression of the P-ST mRNA in the intestine as well as in the liver in the mouse tissues examined (brain, heart, intestine, kidney, liver and lung) of both sexes. E. coli-expressed enzyme is capable of catalyzing the sulfation of 2-naphthol at K(m)=3.3 μM and V(max)=3.33 nmol/min/mg protein and also showed sulfation activity for L-3,4-dihydroxyphenylalanine (L-DOPA) and dopamine. Among food constituents tested, tannic acid and epigallocatechin gallate strongly inhibited the P-ST activity in vitro.

元の言語English
ページ(範囲)234-239
ページ数6
ジャーナルBiological and Pharmaceutical Bulletin
22
発行部数3
DOI
出版物ステータスPublished - 1999 3

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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