Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain

Kentaro Nagamine, Jun Kudoh, Shinsei Minoshima, Kazuhiko Kawasaki, Shuichi Asakawa, Fumiaki Ito, Nobuyoshi Shimizu

研究成果: Article査読

201 被引用数 (Scopus)

抄録

We have isolated cDNA clones for a novel human protein, TRPC7 (transient receptor potential-related channels), which consists of 1503 amino acid residues from the fetal brain and caudate nucleus cDNA libraries. Northern blot analysis indicated that the TRPC7 gene is highly expressed as a 6.5-kb transcript in brain. The TRPC7 protein has significant homology with Caenorhabditis elegans hypothetical proteins T01H8.5, C05C12.3, and F54D1.5 and with Drosophila and human transient receptor potential (trp) proteins. The TRPC7 protein has seven putative transmembrane domains that probably constitute a Ca2+ channel as in the above-mentioned proteins. Genomic sequencing revealed that the TRPC7 gene consists of 32 exons spanning approximately 90 kb. The TRPC7 gene was mapped between D21S400 and D21S171 on human chromosome 21q22.3, 14 kb distal to a NotI site in D21S400. This novel TRPC7 gene could be a candidate gene for genetic disorders such as bipolar affective disorder, nonsyndromic hereditary deafness, Knobloch syndrome, and holoprosencephaly, which were mapped to this region.

本文言語English
ページ(範囲)124-131
ページ数8
ジャーナルGenomics
54
1
DOI
出版ステータスPublished - 1998 11 15

ASJC Scopus subject areas

  • 遺伝学

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