Molecular Cloning of an Amylase Gene of Bacillus circulans

Masafumi Nishizawa, Fumiko Ozawa, Fumio Hishinuma

研究成果: Article

20 引用 (Scopus)

抜粋

An amylase gene of Bacillus circulans was cloned in B. subtilis and its nucleotide sequence was determined. The putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. Homologous regions with other amylases of Bacillus species were found. A σ55-type promoter is located at about 250 bp upstream from the starting codon. This promoter was also functional in Escherichia coli, and able to express β-galactosidase activity.

元の言語English
ページ(範囲)255-265
ページ数11
ジャーナルDNA
6
発行部数3
DOI
出版物ステータスPublished - 1987 6

    フィンガープリント

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

これを引用

Nishizawa, M., Ozawa, F., & Hishinuma, F. (1987). Molecular Cloning of an Amylase Gene of Bacillus circulans. DNA, 6(3), 255-265. https://doi.org/10.1089/dna.1987.6.255