Molecular-cloning of cdna encoding human drebrin e and chromosomal mapping of its gene

Masahiro Toda, Tomoaki Shirao, Shinsei Minoshima, Nobuyoshi Shimizu, Shigeo Toya, Keiichi Uyemura

研究成果: Article査読

28 被引用数 (Scopus)

抄録

Drebrins are novel actin-binding proteins in the brain which are developmentally regulated. Three isoforms: two embryonic types (El and E2) and an adult type (A) are generated by alternative RNA splicing from a single drebrin gene in the chicken brain. A full length cDNA clone of human drebrin E has been isolated from a cDNA library of human fetus brain. The clone is 2596 base pairs in length and contains an open reading frame of 1947 nucleotides encoding a protein of 649 amino acids. The deduced amino acid sequence, except for the internal 138-nucleotide sequence (ins2), exhibits 88% homology with rat drebrin A. Spot blot hybridization using flow-sorted human chromosomes provides evidence that the gene encoding human drebrin protein locates on human chromosome 5.

本文言語English
ページ(範囲)468-472
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
196
1
DOI
出版ステータスPublished - 1993 10 15

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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