TY - JOUR
T1 - Molecular cloning of cDNA encoding human Rab3D whose expression is upregulated with myeloid differentiation
AU - Nishio, Hitoshi
AU - Suda, Toshio
AU - Sawada, Ken Ichi
AU - Miyamoto, Takeshi
AU - Koike, Takao
AU - Yamaguchi, Yuji
N1 - Funding Information:
We are indebted to Drs. Kenji Kishi and Michio Nakamura for providing an HT93A cell line and gp91 phox cDNA, respectively. We are grateful to Dr. Takeshi Naruse for his suggestions. This work was supported by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan.
PY - 1999/2/16
Y1 - 1999/2/16
N2 - To identify genes expressed in myeloid differentiation, we isolated a cDNA fragment by differential display using RNA prepared from HT93A cells, a human cell line capable of differentiating into neutrophil and eosinophil lineages in response to retinoic acid (RA). Evaluation of the full-length clone isolated from an HT93A cDNA library showed that it encoded a 24 kDa protein comprised of several domains conserved in the Ras superfamily. Comparison of the deduced amino acid sequence of this clone with Rab proteins revealed that it had highest homology to a small GTP-binding protein, murine Rab3D. The mRNA expression of human Rab3D was upregulated in the course of myeloid differentiation, and it was preferentially expressed in granulocytes. These results suggest that human Rab3D may play a specific role in granulocytes, for example in exocytosis of neutrophil-specific granules or in degranulation of both eosinophils and basophils.
AB - To identify genes expressed in myeloid differentiation, we isolated a cDNA fragment by differential display using RNA prepared from HT93A cells, a human cell line capable of differentiating into neutrophil and eosinophil lineages in response to retinoic acid (RA). Evaluation of the full-length clone isolated from an HT93A cDNA library showed that it encoded a 24 kDa protein comprised of several domains conserved in the Ras superfamily. Comparison of the deduced amino acid sequence of this clone with Rab proteins revealed that it had highest homology to a small GTP-binding protein, murine Rab3D. The mRNA expression of human Rab3D was upregulated in the course of myeloid differentiation, and it was preferentially expressed in granulocytes. These results suggest that human Rab3D may play a specific role in granulocytes, for example in exocytosis of neutrophil-specific granules or in degranulation of both eosinophils and basophils.
KW - In vitro transcription/translation
KW - Myeloid differentiation
KW - Polymerase chain reaction differential display
KW - Rab3D
KW - cDNA cloning
KW - mRNA expression
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U2 - 10.1016/S0167-4781(98)00279-6
DO - 10.1016/S0167-4781(98)00279-6
M3 - Article
C2 - 10023084
AN - SCOPUS:0033573958
VL - 1444
SP - 283
EP - 290
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 2
ER -